Article

Effect of Palm Tocotrienol Rich Fraction on Oxidative Status of Heart in a Rat Model of Isoprenaline-Induced Myocardial Injury
Vol. 6 Issue 1 : 2018

Abstract

Background: Palm oil tocotrienol rich fraction (TRF) has shown potential as a cardioprotective, antioxidant, anti-inflammation, anti-ageing as well as anticancer agent through inhibiting free radical production and enhancing the antioxidant enzyme activity. Therefore, this study aimed to identify the effect of palm TRF on oxidative status in isoprenaline-induced myocardial injury in rats.
Methods: A total of 24 male Sprague Dawley rats (200-250 g) were randomly divided into control group administered with vitamin E-stripped oil (1 mg/kg body weight; n=12) and TRF-supplemented group (200 mg/kg body weight; n=12), for 84 consecutive days. On the last two days of the treatment, the control group was further divided into control and ISO-induced myocardial injury (MI) group, whereas TRF-supplemented group was divided into TRF and TRF+MI group. MI and TRF+MI groups were injected with isoprenaline (ISO) (85 mg/kg) for two days with the interval of 24 hours to induce MI. Meanwhile, control and TRF groups were injected with normal saline (1 mg/kg).
Results: Heart and left ventricle weight were increased in MI compared to control. Aspartate aminotransferase (AST) and creatine kinase-MB (CK-MB) were increased in MI compared to control. Histology showed the presence of necrosis, cell debris, separation of cardiomyocytes fibre and inflammatory cells in MI and with TRF supplementation. Immunohistochemical staining of macrophage marker, CD68, showed no detection in all groups while immunohistochemical staining for nitrotyrosine showed its presence in ISO-induced MI and with TRF supplementation. No changes of oxidative status were observed in either MI or with TRF supplementation.
Conclusion: TRF supplementation could reduce myocardial injury but not by reducing oxidative damage. Further study is required to examine its exact mechanism.